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pgemhe cherry trim21  (Addgene inc)


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    Structured Review

    Addgene inc pgemhe cherry trim21
    (A) Expression and localization of CCDC66 in different stages of mouse oocyte meiotic maturation. Oocytes were stained with anti-CCDC66 antibody (gray) at (0, 5, 7, and 16) hours of maturation corresponding to Prophase I, Pro-metaphase I, Metaphase I and Metaphase II, respectively. Tubulin and DAPI was used to label the spindle and DNA (green and blue, respectively). (B) Prophase-I arrested oocytes were co-microinjected with <t>Trim21</t> cRNA and either CCDC66 antibody or IgG. Oocytes were fixed at metaphase I and stained to detect CCDC66 (gray). Tubulin (green) and DAPI (blue) were used to label the spindle and DNA. (C) Relative CCDC66 intensity from B, two-tailed unpaired Students t-Test (***p < 0.001). (D) Quantification of percentage of polar body extrusion (PBE). (E) Percentage of aneuploid Metaphase II eggs after knockdown of CCDC66 in young and old mouse oocytes (One-way ANOVA, * p <0.05; ** p < 0.01, *** p < 0.001). Number of oocytes examined: young IgG: 34, young CCDC66: 37, old IgG: 29, old CCDC66: 31. These experiments were repeated 3 times. Scale bars: 15 and 4 μm (insets).
    Pgemhe Cherry Trim21, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgemhe cherry trim21/product/Addgene inc
    Average 93 stars, based on 23 article reviews
    pgemhe cherry trim21 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Identifying risk genes for embryo aneuploidy using ultra-low coverage whole-genome sequencing"

    Article Title: Identifying risk genes for embryo aneuploidy using ultra-low coverage whole-genome sequencing

    Journal: medRxiv

    doi: 10.1101/2023.07.22.23292618

    (A) Expression and localization of CCDC66 in different stages of mouse oocyte meiotic maturation. Oocytes were stained with anti-CCDC66 antibody (gray) at (0, 5, 7, and 16) hours of maturation corresponding to Prophase I, Pro-metaphase I, Metaphase I and Metaphase II, respectively. Tubulin and DAPI was used to label the spindle and DNA (green and blue, respectively). (B) Prophase-I arrested oocytes were co-microinjected with Trim21 cRNA and either CCDC66 antibody or IgG. Oocytes were fixed at metaphase I and stained to detect CCDC66 (gray). Tubulin (green) and DAPI (blue) were used to label the spindle and DNA. (C) Relative CCDC66 intensity from B, two-tailed unpaired Students t-Test (***p < 0.001). (D) Quantification of percentage of polar body extrusion (PBE). (E) Percentage of aneuploid Metaphase II eggs after knockdown of CCDC66 in young and old mouse oocytes (One-way ANOVA, * p <0.05; ** p < 0.01, *** p < 0.001). Number of oocytes examined: young IgG: 34, young CCDC66: 37, old IgG: 29, old CCDC66: 31. These experiments were repeated 3 times. Scale bars: 15 and 4 μm (insets).
    Figure Legend Snippet: (A) Expression and localization of CCDC66 in different stages of mouse oocyte meiotic maturation. Oocytes were stained with anti-CCDC66 antibody (gray) at (0, 5, 7, and 16) hours of maturation corresponding to Prophase I, Pro-metaphase I, Metaphase I and Metaphase II, respectively. Tubulin and DAPI was used to label the spindle and DNA (green and blue, respectively). (B) Prophase-I arrested oocytes were co-microinjected with Trim21 cRNA and either CCDC66 antibody or IgG. Oocytes were fixed at metaphase I and stained to detect CCDC66 (gray). Tubulin (green) and DAPI (blue) were used to label the spindle and DNA. (C) Relative CCDC66 intensity from B, two-tailed unpaired Students t-Test (***p < 0.001). (D) Quantification of percentage of polar body extrusion (PBE). (E) Percentage of aneuploid Metaphase II eggs after knockdown of CCDC66 in young and old mouse oocytes (One-way ANOVA, * p <0.05; ** p < 0.01, *** p < 0.001). Number of oocytes examined: young IgG: 34, young CCDC66: 37, old IgG: 29, old CCDC66: 31. These experiments were repeated 3 times. Scale bars: 15 and 4 μm (insets).

    Techniques Used: Expressing, Staining, Two Tailed Test



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    pgemhe cherry trim21  (Addgene inc)
    93
    Addgene inc pgemhe cherry trim21
    (A) Expression and localization of CCDC66 in different stages of mouse oocyte meiotic maturation. Oocytes were stained with anti-CCDC66 antibody (gray) at (0, 5, 7, and 16) hours of maturation corresponding to Prophase I, Pro-metaphase I, Metaphase I and Metaphase II, respectively. Tubulin and DAPI was used to label the spindle and DNA (green and blue, respectively). (B) Prophase-I arrested oocytes were co-microinjected with <t>Trim21</t> cRNA and either CCDC66 antibody or IgG. Oocytes were fixed at metaphase I and stained to detect CCDC66 (gray). Tubulin (green) and DAPI (blue) were used to label the spindle and DNA. (C) Relative CCDC66 intensity from B, two-tailed unpaired Students t-Test (***p < 0.001). (D) Quantification of percentage of polar body extrusion (PBE). (E) Percentage of aneuploid Metaphase II eggs after knockdown of CCDC66 in young and old mouse oocytes (One-way ANOVA, * p <0.05; ** p < 0.01, *** p < 0.001). Number of oocytes examined: young IgG: 34, young CCDC66: 37, old IgG: 29, old CCDC66: 31. These experiments were repeated 3 times. Scale bars: 15 and 4 μm (insets).
    Pgemhe Cherry Trim21, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgemhe cherry trim21/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    pgemhe cherry trim21 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Expression and localization of CCDC66 in different stages of mouse oocyte meiotic maturation. Oocytes were stained with anti-CCDC66 antibody (gray) at (0, 5, 7, and 16) hours of maturation corresponding to Prophase I, Pro-metaphase I, Metaphase I and Metaphase II, respectively. Tubulin and DAPI was used to label the spindle and DNA (green and blue, respectively). (B) Prophase-I arrested oocytes were co-microinjected with Trim21 cRNA and either CCDC66 antibody or IgG. Oocytes were fixed at metaphase I and stained to detect CCDC66 (gray). Tubulin (green) and DAPI (blue) were used to label the spindle and DNA. (C) Relative CCDC66 intensity from B, two-tailed unpaired Students t-Test (***p < 0.001). (D) Quantification of percentage of polar body extrusion (PBE). (E) Percentage of aneuploid Metaphase II eggs after knockdown of CCDC66 in young and old mouse oocytes (One-way ANOVA, * p <0.05; ** p < 0.01, *** p < 0.001). Number of oocytes examined: young IgG: 34, young CCDC66: 37, old IgG: 29, old CCDC66: 31. These experiments were repeated 3 times. Scale bars: 15 and 4 μm (insets).

    Journal: medRxiv

    Article Title: Identifying risk genes for embryo aneuploidy using ultra-low coverage whole-genome sequencing

    doi: 10.1101/2023.07.22.23292618

    Figure Lengend Snippet: (A) Expression and localization of CCDC66 in different stages of mouse oocyte meiotic maturation. Oocytes were stained with anti-CCDC66 antibody (gray) at (0, 5, 7, and 16) hours of maturation corresponding to Prophase I, Pro-metaphase I, Metaphase I and Metaphase II, respectively. Tubulin and DAPI was used to label the spindle and DNA (green and blue, respectively). (B) Prophase-I arrested oocytes were co-microinjected with Trim21 cRNA and either CCDC66 antibody or IgG. Oocytes were fixed at metaphase I and stained to detect CCDC66 (gray). Tubulin (green) and DAPI (blue) were used to label the spindle and DNA. (C) Relative CCDC66 intensity from B, two-tailed unpaired Students t-Test (***p < 0.001). (D) Quantification of percentage of polar body extrusion (PBE). (E) Percentage of aneuploid Metaphase II eggs after knockdown of CCDC66 in young and old mouse oocytes (One-way ANOVA, * p <0.05; ** p < 0.01, *** p < 0.001). Number of oocytes examined: young IgG: 34, young CCDC66: 37, old IgG: 29, old CCDC66: 31. These experiments were repeated 3 times. Scale bars: 15 and 4 μm (insets).

    Article Snippet: Rabbit anti-CCDC66 antibody (Bethyl Laboratories, #A303-339A) and control IgG antibody (Merck Millipore, #12-370) were purified using Amicon Ultra 0.5-ml Centrifugal Filter (Merk Millipore, #UFC5003096). pGEMHE-Cherry-TRIM21 (Addgene, #105522) or pGEMHE-mEGFP-mTrim21 (Addgene, #105519) were linearized with Asc I (New England Biolabs, #R0558S,) and in vitro transcribed using a T7 mMessage mMachine Kit (Ambion, #AM1340).

    Techniques: Expressing, Staining, Two Tailed Test